FastQCFastQC Report
Thu 2 Jun 2016
DRR028571_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028571_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960449
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16000.053.824634
ATCAACG16650.051.7233733
GACGACC22250.048.363113145
CAACGCA19200.045.2316365
GTATCAA22150.042.1750221
AACGCAG22700.038.2556086
ACGCAGA23000.037.1201257
CGCAGAG22500.036.968238
GGTATCA14050.034.803071
TATCAAC26050.034.1799282
GTACATG33300.033.313361
TACATGG35650.029.070192
ACATGGG41200.023.0284773
GCAGAGT34000.020.815019
GTATTAG15850.020.2601431
TCTGTCG6250.019.8461048
GAGTACA29650.019.4456061
GTACCGG2000.007626080418.2437466
AGACCGT2400.001014726118.2437466
GTATAGG9250.018.1470151
Produced by FastQC (version 0.11.5)