FastQCFastQC Report
Thu 2 Jun 2016
DRR028571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960449
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.064.012541
GTATCAA34750.049.3811761
ATCAACG35350.046.3023453
TCAACGC35250.046.0227784
CAACGCA36800.044.0843165
AACGCAG38650.041.97426
TATCAAC44300.037.601752
ACGCAGA44900.035.8088657
CGCAGAG45250.035.0517278
GTACATG35600.033.904891
TACATGG38100.028.893662
ACATGGG37550.026.6166293
GCAGAGT58500.026.1222449
CAGAGTA54950.020.956249
GTGGTAT14100.020.6274281
TATACTG15700.020.297245
GAGTACA29850.019.97441
TGGTATC13800.018.3684652
CATGGGG29950.017.6526534
TATACAG14900.017.4984725
Produced by FastQC (version 0.11.5)