FastQCFastQC Report
Thu 2 Jun 2016
DRR028570_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028570_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847187
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17400.052.826484
ATCAACG17800.051.229533
CAACGCA19950.045.7085535
GACGACC22900.045.135284145
AACGCAG21700.042.357396
GTATCAA24050.040.3428841
ACGCAGA23550.038.7201967
CGCAGAG23500.038.4889838
GGTATCA14650.038.342771
TATCAAC26500.034.686072
GTACATG38300.032.761221
TACATGG40650.028.175362
ACATGGG42300.026.3864783
GAGTACA33750.026.3703841
GCAGAGT33750.023.3417059
AGTACAT35450.022.4305882
GTATAAG11850.019.6998081
CATGGGG30000.019.4535624
GTATAGA11700.018.0818371
TTATACT19300.017.7652344
Produced by FastQC (version 0.11.5)