FastQCFastQC Report
Thu 2 Jun 2016
DRR028570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847187
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.068.1216661
GTATCAA40350.054.6142851
ATCAACG40150.054.116383
TCAACGC40300.053.9149484
CAACGCA43050.050.470915
AACGCAG44700.048.7699136
TATCAAC48950.044.6835062
ACGCAGA51300.042.071887
CGCAGAG52350.040.9513328
GTACATG38750.036.599071
GCAGAGT61950.032.851719
TACATGG39800.032.573392
ACATGGG41250.029.1458783
GTGGTAT13950.027.1104241
TCTGTCG5050.022.9467748
CATACCG1600.00218883822.6330475
TGGTATC14300.022.2848452
ACCGTCC1700.00311221521.3016938
CATGGGA20500.020.8444844
CATGGGG29900.020.589264
Produced by FastQC (version 0.11.5)