FastQCFastQC Report
Thu 2 Jun 2016
DRR028569_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028569_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815368
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17900.046.8939364
ATCAACG18350.045.7439543
GACGACC21950.044.437225145
CAACGCA20250.041.450765
GGTATCA13350.041.0064241
GTATCAA22050.040.0542531
AACGCAG21400.038.8800166
GTACATG38850.034.3820651
CGCAGAG24000.033.4459658
ACGCAGA24050.033.381117
TATCAAC27500.031.3199622
TACATGG42400.030.814782
ACATGGG45100.027.3515573
GTATAGG6500.026.9506841
TATTACG2451.8060473E-623.8312647
GTCTAAG5250.022.2450081
GTATACG2353.5228288E-521.7421281
GTCGTAC1750.003519337220.8546941
GAGTACA33200.020.6662481
GCAGAGT35950.020.5014579
Produced by FastQC (version 0.11.5)