FastQCFastQC Report
Thu 2 Jun 2016
DRR028569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815368
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.073.542161
GTATCAA37700.050.3404541
ATCAACG40450.046.377713
TCAACGC41100.045.8204734
CAACGCA43050.043.7449845
AACGCAG45900.040.8709876
TATCAAC47250.040.1631282
CGCAGAG50900.035.8600438
ACGCAGA51600.035.6543167
GTACATG35800.033.3103181
TACATGG39700.029.1915762
GCAGAGT62350.028.3453249
TGGTATC11400.026.049962
GTGGTAT12100.025.8405081
ACATGGG44700.024.4679433
CAGAGTA60200.022.4995169
ATACCGC2256.914417E-419.3150927
GAGTACA33700.019.2033961
TATACTG11750.019.1096135
GTATTAG17950.019.0393161
Produced by FastQC (version 0.11.5)