FastQCFastQC Report
Thu 2 Jun 2016
DRR028568_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028568_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114591
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32750.059.0591851
GTATCAA56200.053.3775251
TCAACGC56500.051.1563644
ATCAACG58200.049.66213
GTACATG113200.044.3603441
CAACGCA67000.043.2468455
TATCAAC69250.042.7916642
GACGACC44850.041.575512145
TACATGG117750.041.2825242
AACGCAG71050.040.987136
ACGCAGA74450.038.036337
CGCAGAG75900.037.3060268
ACATGGG127250.036.4224243
CATGGGA69600.033.557814
GCAGAGT88600.029.569419
GAGTACA85450.024.5144821
GTACGGA4550.024.0612726
CATGGGG63850.024.0055314
AGTACAT84800.023.067042
GTGGTAT20350.022.2372041
Produced by FastQC (version 0.11.5)