FastQCFastQC Report
Thu 2 Jun 2016
DRR028568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114591
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37080.11905254975693438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35610.11433282893323712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48100.082.537811
GTATCAA87450.062.0275151
TCAACGC103600.051.6595424
ATCAACG105250.050.9872933
CAACGCA111300.048.085615
TATCAAC113300.047.556392
AACGCAG115300.046.4802326
ACGCAGA128750.041.455887
GTACATG116200.040.9864731
CGCAGAG129800.040.6741688
TACATGG122200.038.2256052
ACATGGG123300.036.2987173
GCAGAGT145400.034.4175159
CATGGGA71700.033.5339554
GTGGTAT23950.030.3598651
GTACGGA5300.027.3287646
TGGTATC25550.026.9276562
GAGTACA89750.025.8441121
TTTCGCG4001.8189894E-1225.3474279
AGTACAT88200.024.715182
Produced by FastQC (version 0.11.5)