FastQCFastQC Report
Thu 2 Jun 2016
DRR028567_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028567_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664295
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG17400.053.2625963
TCAACGC17600.052.657344
GGTATCA13100.049.577721
CAACGCA19300.048.0161935
AACGCAG20250.045.403246
GTATCAA22950.043.2438241
GACGACC20500.038.844105145
CGCAGAG23450.038.2704628
ACGCAGA23800.037.709977
TATCAAC26400.035.6577262
GTACATG35000.030.2320631
GCAGAGT31350.026.0656579
TACATGG37900.025.6082732
GTCTTAC6950.023.0996671
ACATGGG41900.021.2477863
GTATTAG14500.021.1372811
AGGCCGT3207.9680103E-720.5226696
CTAGACG1800.004148741320.2705424
CGGCTCG1900.00567788519.200159
ATACGGG3852.2313725E-718.9525367
Produced by FastQC (version 0.11.5)