FastQCFastQC Report
Thu 2 Jun 2016
DRR028567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664295
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19440.11680621524429262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19270.11578476171592177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.083.9195861
GTATCAA39450.057.1603131
TCAACGC45200.049.6699834
ATCAACG45100.049.6195343
CAACGCA46750.048.178085
AACGCAG48900.046.3560266
TATCAAC51850.043.4392552
ACGCAGA55450.040.4884227
CGCAGAG56050.039.9257978
GCAGAGT67600.031.2828929
GTACATG34350.030.070561
GTGGTAT13300.029.5339831
TGGTATC12400.029.2024352
ACCGCGT1050.007750482827.589358
TCTAGCG1358.1359496E-426.8229772
TAGCGCA1100.00972425826.3352854
GTACGGA2207.561666E-726.3352856
TACATGG37800.025.0986422
ACATGGG38050.023.2207323
CAGAGTA64650.021.508179
Produced by FastQC (version 0.11.5)