FastQCFastQC Report
Thu 2 Jun 2016
DRR028566_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028566_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849325
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.051.815561
GACGACC19550.046.247444145
ATCAACG17700.044.1086463
TCAACGC17750.043.9843984
GTATCAA22700.037.285961
CAACGCA21850.036.062985
GGGTACG1501.0792864E-634.0502241
AACGCAG23750.032.870746
GTACATG35550.031.8130511
ACGCAGA24900.031.0595997
CGCAGAG24000.031.004918
TACATGG36150.028.8629632
TATCAAC29150.028.28482
ACATGGG37100.026.7471923
TACCGTC1409.634884E-426.0574137
GTACGAC1450.00118196725.1602653
GGTACGA1801.456425E-424.321592
TAGACCG1550.001743590823.5357275
GAGTACA29150.022.7780251
ATACCGT2754.9361315E-621.2249456
Produced by FastQC (version 0.11.5)