FastQCFastQC Report
Thu 2 Jun 2016
DRR028566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1849325
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19010.10279426277155178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18590.100523163857083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24200.073.9006651
GTATCAA43250.059.3358541
ATCAACG46850.053.7932363
TCAACGC46850.053.7932364
CAACGCA49000.051.2851225
AACGCAG51400.048.8904886
TATCAAC55650.045.5471152
CGCAGAG59050.042.3113828
ACGCAGA59450.042.148517
TAGACCG1157.200395E-637.78435
GCAGAGT70450.034.539529
GTACATG36650.033.324521
TACATGG37700.028.43012
TACCGTC1306.5270904E-427.8538137
TGCACCG2259.202813E-725.7493045
TGTACCG1701.039535E-425.559975
ACATGGG40400.025.096013
GTGGTAT14100.024.2330071
ACCGTCC1500.001504403424.139978
GTACGGA1851.8452555E-423.487546
Produced by FastQC (version 0.11.5)