FastQCFastQC Report
Thu 2 Jun 2016
DRR028565_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028565_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814363
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.055.1981541
TCAACGC21650.045.84324
ATCAACG21800.045.527773
GTATCAA24900.043.0835951
GACGACC22750.041.62434145
GTACATG48000.041.0503161
CAACGCA27100.036.892075
AACGCAG28550.035.529616
TATCAAC28950.035.0396842
TACATGG52450.034.9238552
ACGCAGA28600.034.1897667
CGCAGAG29050.032.3987248
ACATGGG56400.031.4427933
GTACGGA3056.6029315E-1026.3193176
CATGGGA26800.026.1414934
GTATTAG18200.024.459771
TATAGCG1952.5020135E-422.4548724
GCAGAGT41200.021.604039
GAGTACA39900.021.2167591
TATTAGG16550.021.165922
Produced by FastQC (version 0.11.5)