FastQCFastQC Report
Thu 2 Jun 2016
DRR028565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814363
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23300.081.140661
GTATCAA45750.061.509331
TCAACGC51600.053.060634
ATCAACG52150.052.639923
CAACGCA55000.049.517135
TATCAAC56950.048.457562
AACGCAG57600.047.4077346
ACGCAGA62900.042.7221957
CGCAGAG64300.041.7920078
GTACATG47900.036.584971
GCAGAGT78250.032.8605089
TACATGG51200.031.8304562
ACATGGG51700.030.6820143
TAGATCG2105.031343E-727.5931325
GTGGTAT13150.027.0951441
CATGGGA26500.027.0594924
TGGTATC12050.027.0492842
GTACCGT1100.00971798326.33896
CAGAGTA70050.024.8160939
TTTCGCG2651.3901808E-724.5995379
Produced by FastQC (version 0.11.5)