FastQCFastQC Report
Thu 2 Jun 2016
DRR028564_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028564_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727947
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14950.051.7225571
ATCAACG20850.044.0838743
TCAACGC21300.043.152534
GTACATG44100.040.8576771
GACGACC19100.040.588055145
GTATCAA24700.039.870541
CAACGCA24150.038.0588725
TACATGG45750.035.8762252
AACGCAG26750.034.359696
ACATGGG48200.031.7824633
TATCAAC30050.031.3155842
ACGCAGA28050.031.2060017
CGCAGAG27850.030.1187448
TAGCTCG1409.6508407E-426.0499069
CATGGGG31450.025.9784154
CATGGGA21150.025.178384
GAGTACA34500.024.7389971
AGTACAT35400.022.4614892
GTGGTAT11200.022.1450251
GCAGAGT34700.022.0711029
Produced by FastQC (version 0.11.5)