FastQCFastQC Report
Thu 2 Jun 2016
DRR028564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727947
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.12459872901194308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.11886938661891829No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.078.130871
GTATCAA47300.055.64931
ATCAACG50850.050.4269333
TCAACGC51000.050.2786184
CAACGCA52850.048.655695
AACGCAG56750.044.929036
TATCAAC59400.043.2904552
ACGCAGA63050.040.095037
CGCAGAG63350.039.905168
GTACATG43450.037.4861221
TACATGG43900.034.485142
GCAGAGT72600.032.825369
ACATGGG46450.031.032563
CGCTATA1204.0805456E-430.1813742
GTGGTAT13400.029.8449041
TGGTATC14850.025.3645482
CATGGGG31500.024.3750514
CATGGGA22000.024.36464
CAGAGTA69400.022.4403299
GTACGGA3355.5568307E-821.6224776
Produced by FastQC (version 0.11.5)