FastQCFastQC Report
Thu 2 Jun 2016
DRR028563_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028563_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789743
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.063.1422421
GTATCAA28950.052.668641
TCAACGC28550.051.617824
ATCAACG29000.051.0684243
GTACATG51700.045.4380531
TACATGG51650.044.2108042
TATCAAC34800.043.185942
CAACGCA36200.040.7096375
GACGACC27900.040.390297145
ACGCAGA36700.039.3565037
AACGCAG38700.037.8902246
CGCAGAG37750.036.9049388
ACATGGG64350.033.5581173
CATGGGG41200.032.758874
GCAGAGT43950.028.5430669
GAGTACA41800.026.3545171
CATGGGA23600.024.730474
AGTACGG3001.5990736E-824.3182965
AGTACAT40800.024.1394862
GTACCGC1952.5079245E-422.4470216
Produced by FastQC (version 0.11.5)