FastQCFastQC Report
Thu 2 Jun 2016
DRR028563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789743
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.10068484693053696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.086.281991
GTATCAA46000.060.5466961
ATCAACG54200.050.385063
TCAACGC54600.050.0159384
TATCAAC59700.045.7432172
CAACGCA60700.045.108965
AACGCAG64550.042.5307126
GTACATG51950.042.133781
TACATGG51550.041.1716882
ACGCAGA69900.038.8609857
CGCAGAG70350.038.2005428
ACATGGG58550.035.259623
GTGGTAT11300.034.107271
GCAGAGT78000.033.6181379
TGGTATC12750.027.8384842
CATGGGA23650.027.565834
CATGGGG43900.027.2257044
TAACGCA2207.5486787E-726.3406834
TAGCGTT1500.00150233524.1456244
CAGAGTA75050.023.9365049
Produced by FastQC (version 0.11.5)