FastQCFastQC Report
Thu 2 Jun 2016
DRR028562_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028562_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006841
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.055.8634341
ATCAACG25300.045.870843
TCAACGC25400.045.4028854
GTACATG49850.042.9005171
GTATCAA29250.041.423071
GACGACC26900.039.987442145
CAACGCA30300.038.06055
TACATGG51950.037.2323462
AACGCAG32800.035.382086
ACGCAGA31950.034.9526947
ACATGGG53600.034.7244573
CGCAGAG32750.034.0980268
TATCAAC35650.033.3724562
CATGGGG38700.026.215854
GCAGAGT43000.024.2720039
CATGGGA25700.023.2884624
GTATATA13600.020.9307981
GTACGGA2805.750411E-620.8541286
GAGTACA42000.020.506561
TACACGC3651.2685814E-719.9971085
Produced by FastQC (version 0.11.5)