FastQCFastQC Report
Thu 2 Jun 2016
DRR028562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006841
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.070.138641
GTATCAA44050.057.4492261
ATCAACG47850.051.924433
TCAACGC48100.051.353364
CAACGCA50550.048.864425
AACGCAG53350.046.2998436
TATCAAC56250.044.556712
ACGCAGA59050.041.4625857
CGCAGAG60100.040.256098
GCAGAGT69350.033.9466139
GTACATG46600.030.117731
GTGGTAT12950.029.2001081
CTTATCG1000.006101815528.9747439
TACATGG48850.027.877442
GTACGGA1907.2087623E-626.6872626
ACATGGG51550.024.7311083
TGGTATC14900.023.8215142
GTATATA14350.023.8175351
ACACCGT5500.023.7066086
GAGTACA41100.022.293571
Produced by FastQC (version 0.11.5)