FastQCFastQC Report
Thu 2 Jun 2016
DRR028561_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028561_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056457
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18800.046.1903084
ATCAACG19550.045.164833
GACGACC25100.042.302616145
GGTATCA17600.042.2911261
CAACGCA22000.038.807375
AACGCAG24000.035.572546
GTATCAA26900.035.2657241
GTACATG37250.033.6948781
ACGCAGA25100.033.13987
CGCAGAG26100.031.5889538
TATCAAC28900.031.3101832
TACATGG41300.029.1538312
ACATGGG46250.023.5091153
TACACGC3203.1650416E-822.8034765
GCAGAGT35900.020.3226979
ACTTACG3259.2579467E-720.2048878
GTATACG2907.805185E-620.130461
GAGTACA34600.020.035921
GTATTAC13500.019.9999851
GCGGGTA2205.660657E-419.8977689
Produced by FastQC (version 0.11.5)