FastQCFastQC Report
Thu 2 Jun 2016
DRR028561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056457
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.069.2929461
GTATCAA42400.051.5986981
TCAACGC42350.048.3884774
ATCAACG42650.048.0481153
CAACGCA46550.044.02265
AACGCAG48600.042.1656766
TATCAAC52300.039.7364542
ACGCAGA54950.036.765927
CGCAGAG54750.036.371198
GTACATG38550.035.823441
GTGGTAT14100.031.44481
TACATGG42550.028.9306562
GCAGAGT65450.028.876189
ACATGGG45700.025.0351283
TGGTATC16600.023.9918372
GAGTACA36900.020.879381
CAGAGTA63050.020.098429
CCTATAC9150.019.7846243
TATACAG14000.019.1373845
TCTGTCG8350.019.0785398
Produced by FastQC (version 0.11.5)