FastQCFastQC Report
Thu 2 Jun 2016
DRR028560_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028560_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803035
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13300.043.2808575
GGTATCA14000.042.158931
ATCAACG14300.040.2553564
CAACGCA15750.036.548286
AACGCAG16800.034.264017
GTACATG34450.033.842571
TATGCCG1153.0712233E-431.6805385
TACATGG35550.030.5407222
GTATCAA20800.029.4271741
ACGCAGA19150.028.9161728
TCTGTCG5200.028.0235168
ACATGGG36700.027.9953333
CGCAGAG19300.027.9356129
TATCAAC22500.026.8799343
TCCGCGC1958.428075E-626.1552838
TCTCGGG22250.024.560816145
CTGTCGC5800.023.8676329
GTATATA16200.023.8392981
CCGCGCG2053.5476062E-421.3246129
AGTACGG2754.99236E-621.197165
Produced by FastQC (version 0.11.5)