FastQCFastQC Report
Thu 2 Jun 2016
DRR028560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803035
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.053.7119331
TCAACGC25300.051.5307464
ATCAACG26900.048.1964653
CAACGCA26950.048.1070485
GTATCAA28950.047.2225461
AACGCAG28200.045.9746446
TTTCGCG1151.4243051E-744.0874189
TATCAAC33700.039.760912
ACGCAGA33350.038.4407547
CGCAGAG33900.037.389778
GTACATG35650.031.004461
TACATGG37150.028.8547522
GTACGGA2654.611138E-927.3318216
GCAGAGT45950.026.9541139
ACATGGG38550.025.3643553
TAGCACG1952.6331906E-422.2859464
AGTACGG3355.5621058E-821.6206955
CGTATAC2053.692257E-421.1988263
GTATATA15950.020.9718761
GCGTCAG3508.4732164E-820.7764851
Produced by FastQC (version 0.11.5)