FastQCFastQC Report
Thu 2 Jun 2016
DRR028559_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028559_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856178
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18500.059.0734024
ATCAACG19750.055.3345763
GTATCAA23450.050.3320431
CAACGCA22700.048.463155
GGTATCA14050.044.595861
AACGCAG25650.043.1699186
TATCAAC26350.042.857172
ACGCAGA25850.041.4268387
CGCAGAG25650.041.1773348
GTAACGG1051.8015284E-434.6938973
GTACATG42550.032.0194931
TACATGG46300.028.167252
CCGGTCG2903.7471182E-1027.6294549
GTATATA12600.024.863961
GCAGAGT37250.024.8344769
GTATAGG9400.024.0273721
ACATGGG49300.023.4975513
TCTCGGG27150.023.050188145
GAGTACA34950.021.2630421
CGACGAC8250.020.925814145
Produced by FastQC (version 0.11.5)