FastQCFastQC Report
Thu 2 Jun 2016
DRR028559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856178
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.060.1127361
TCAACGC28850.052.477854
GTATCAA30200.051.518671
ATCAACG29550.050.9895823
CAACGCA31250.048.2157485
AACGCAG33700.044.710456
TATCAAC36800.040.944082
ACGCAGA38050.039.2182467
CGCAGAG37900.038.8000648
TTTCGCG1458.7607623E-734.9708069
GTACATG39800.033.6118621
TACATGG43950.029.5032422
GCAGAGT50250.026.6692779
ACATGGG47700.025.5132923
GTATAGG8650.022.6937081
GTACGGA2003.1219833E-421.731866
GTATTAG14450.020.6287921
GTATTAA13500.019.9262581
GAGTACA35150.019.236031
CAGAGTA45600.019.2218939
Produced by FastQC (version 0.11.5)