FastQCFastQC Report
Thu 2 Jun 2016
DRR028558_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028558_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569634
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18250.049.1341364
ATCAACG18800.047.308923
GGTATCA14350.044.706621
GTATCAA22050.043.6421781
CAACGCA21700.040.9838835
GTACATG38050.039.4687731
AACGCAG24150.036.5242426
TACATGG38800.036.4511382
TATCAAC25900.035.7474482
ACGCAGA25200.034.7131237
CGCAGAG25200.033.5527658
ACATGGG39800.032.238193
TTTCGCG1804.510808E-628.3453859
CATGGGA18850.024.7519654
GCAGAGT31550.024.4885659
TATAACG1500.001447244824.3007584
GAGTACA29200.024.2175371
ACGATCC8500.023.670519145
TCTCGGG19850.022.805979145
AGTACAT29800.022.751382
Produced by FastQC (version 0.11.5)