FastQCFastQC Report
Thu 2 Jun 2016
DRR028558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569634
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.061.5091061
GTATCAA30200.058.2360731
TCAACGC33500.051.452364
ATCAACG34600.049.816593
CAACGCA35700.048.4844865
AACGCAG37100.047.04536
TATCAAC41100.042.4666822
GTACATG34000.040.398631
ACGCAGA42650.039.9045037
CGCAGAG42550.039.3174678
TACATGG34650.037.831052
GCAGAGT49900.031.639469
ACATGGG39050.031.342873
GTGGTAT11750.027.8328951
GTCGTAC1801.4961815E-424.2249241
TGGTATC12200.023.745062
CGCCGAA1550.001820246123.362076145
CATGGGG26400.023.317834
CAGAGTA48700.022.6041269
CATGGGA19950.022.1442034
Produced by FastQC (version 0.11.5)