FastQCFastQC Report
Thu 2 Jun 2016
DRR028557_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028557_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781084
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37800.048.7970851
GGTATCA24800.046.7423551
ATCAACG37300.046.714773
TCAACGC37600.045.954254
GTACATG74650.039.846991
TACATGG71300.038.447092
TATCAAC49050.036.7132762
CAACGCA49600.034.836285
CGCAGAG48450.034.3025978
ACGCAGA49550.034.1326377
AACGCAG52000.033.0876466
ATCTACC27250.031.113928145
ACATGGG84250.030.8067043
CATGGGG58450.026.3186174
GCAGAGT58650.025.1036939
GAGTACA58200.022.297811
CGACGAC14950.022.108334145
AGTACAT56250.021.7746872
CATGGGA35950.020.482724
TCTCGGG51750.020.410154145
Produced by FastQC (version 0.11.5)