FastQCFastQC Report
Thu 2 Jun 2016
DRR028557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781084
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38300.073.855521
GTATCAA62900.058.4967841
ATCAACG72950.049.6382523
TCAACGC73600.049.1998674
CAACGCA81900.044.3906565
TATCAAC82100.044.370732
AACGCAG86150.042.1166846
CGCAGAG91900.038.4570588
ACGCAGA92800.038.396257
GTACATG71800.035.6492461
TACATGG71850.034.4723632
GCAGAGT103550.032.3119859
ACATGGG77500.030.464053
GTGGTAT16700.027.8673481
TTTCGCG6400.026.0267319
CATGGGG57550.025.0426044
CAGAGTA98300.023.1338489
TGGTATC21100.021.9669232
GTACGGA4058.1854523E-1021.458436
GTATAGA14550.020.990291
Produced by FastQC (version 0.11.5)