FastQCFastQC Report
Thu 2 Jun 2016
DRR028556_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028556_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747420
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC19250.052.253054
ATCAACG19550.051.4512183
CAACGCA22000.045.7214165
GTATCAA23000.045.318081
GGTATCA14850.044.6661221
AACGCAG25050.040.736496
TATCAAC27600.036.444612
GTACATG43350.035.814081
ACGCAGA29000.032.923917
TACATGG45650.031.9339432
CGCAGAG29700.031.9006658
ACATGGG48150.028.4593433
TAGTACG2705.2605174E-926.9960084
CGACGAC7450.026.047266145
TCTCGGG22700.024.379204145
GTACGGA2903.1234958E-722.6207926
AGAGTAC29050.022.080045
GAGTACA33400.022.0413531
CATGGGG34600.021.6982364
GCAGAGT38150.021.3955659
Produced by FastQC (version 0.11.5)