FastQCFastQC Report
Thu 2 Jun 2016
DRR028556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747420
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.065.1507341
GTATCAA37450.053.5837021
ATCAACG37350.053.3195883
TCAACGC37350.053.3195884
CAACGCA39700.050.163395
AACGCAG41700.047.7574736
TATCAAC46350.043.278752
ACGCAGA48000.040.5840847
CGCAGAG48600.039.9340368
GTACATG43850.033.4932441
TACATGG44650.031.4647982
GCAGAGT59800.031.0015549
ACATGGG47350.027.5294283
TAGACCG1358.138963E-426.8213675
GTCGTAC1500.001470118624.2356261
GAGTACA34750.022.1782111
TACACTG13800.022.0401675
CAGAGTA53850.021.7858249
CATGGGA21400.020.9808254
GTGGTAT11800.020.9494361
Produced by FastQC (version 0.11.5)