FastQCFastQC Report
Thu 2 Jun 2016
DRR028555_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028555_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275984
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG11350.048.7897833
TCAACGC11550.048.575794
GGTATCA10600.041.930991
CAACGCA13400.041.324035
GTATCAA15800.040.1211431
AACGCAG14550.037.555626
GTACATG22500.034.650731
ACGCAGA15800.033.1997537
CGCAGAG16450.031.884118
CGACGAC3900.031.352705145
TATCAAC18400.030.8878692
TACATGG22900.030.8636592
TATAGCG1000.00593178129.1420027
GCGGGTA1255.01513E-429.1362179
TGACGCG1050.00801367427.400684145
ACATGGG26850.025.7804473
TCTCGGG14300.023.640272145
TCGCCGG9900.022.52253145
GCAGAGT22500.021.6902969
TAGATCG1800.004185656620.2391095
Produced by FastQC (version 0.11.5)