FastQCFastQC Report
Thu 2 Jun 2016
DRR028555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275984
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.10337120214673537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.072.065941
GTATCAA29500.053.2466281
ATCAACG31300.049.526963
TCAACGC31650.048.979274
CAACGCA32850.047.1900755
AACGCAG34500.044.9331556
TATCAAC37150.041.7279662
ACGCAGA38950.039.4276287
CGCAGAG39050.039.3266648
GTGGTAT10800.034.340511
TGGTATC10300.031.6480922
GCAGAGT47000.031.5957169
ATACCGC1255.1800977E-428.9755887
TATACCG1555.5332566E-528.040896
GTACATG22250.027.781091
ACCGTAC1100.009712351526.3414448
TACATGG23000.024.8812082
TGGACCG1751.2631933E-424.8362185
TCTGTCG3851.6370905E-1124.459918
TAGATCG1500.001501845724.1463245
Produced by FastQC (version 0.11.5)