FastQCFastQC Report
Thu 2 Jun 2016
DRR028554_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028554_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551262
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.052.6905331
TCAACGC16800.047.2907944
ATCAACG17150.047.1756973
GTATCAA20100.041.7024151
CAACGCA19800.039.7561045
GTACATG34450.038.507171
AACGCAG21450.037.0365266
TATCAAC23500.033.497732
TACATGG38600.033.4229972
ACGCAGA23650.032.974927
CGCAGAG23100.032.495858
TCTCGGG19800.029.767668145
ACATGGG43400.028.2149313
CATGGGA17800.024.5691814
GCAGAGT31200.022.8914959
GTACGGA1600.00210992122.776196
ATAATAC13050.021.2242583
GTATTAG15350.018.9937651
CGTAGAG1950.00663431918.6893793
AGTACGG1950.006635571418.6887665
Produced by FastQC (version 0.11.5)