FastQCFastQC Report
Thu 2 Jun 2016
DRR028554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551262
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18750.079.895681
GTATCAA33300.056.560461
ATCAACG36400.051.5400283
TCAACGC36950.050.576824
CAACGCA39650.047.132755
AACGCAG41450.045.2607236
TATCAAC43550.043.2445562
ACGCAGA46250.039.9369357
CGCAGAG46250.039.6237038
ACGTGCG850.002754603734.0868878
TAGATCG1751.0178337E-733.1129725
GTACATG35400.032.4572141
GCAGAGT57450.030.8903129
GTATAGG7450.028.3073441
TACATGG41250.027.0422652
GTACGGA2051.2999168E-524.7337766
TTTCGCG1500.001502515524.1448769
ACATGGG45300.023.8250793
TACGTGC1550.001818453923.366017
TGGTATC9350.022.4663562
Produced by FastQC (version 0.11.5)