FastQCFastQC Report
Thu 2 Jun 2016
DRR028553_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028553_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551220
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA11020.19992017706179024No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA9740.1766989586734879No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA9540.17307064330031566No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA8600.15601756104640616No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC8570.15547331374043033No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG8380.1520264141359167No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG8070.14640252530749973No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG7860.1425927941656689No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC7710.1398715576357897No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA7680.13932731032981388No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG7580.13751315264322775No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT7300.1324335111207866No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA7200.1306193534342005No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA7170.13007510612822465No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG6880.12481404883712492No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT6470.11737600232212184No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA6440.11683175501614601No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG6420.11646892347882878No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC6400.11610609194151157No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT6390.11592467617285294No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA6310.11447335002358405No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT6130.11120786618772904No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT6080.11030078734443599No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA6030.10939370850114292No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA6020.10921229273248431No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC5850.10612822466528792No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG5800.10522114582199485No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC5690.1032255723667501No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA5640.10231849352345707No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAC259.1903727E-487.458823
GCTCTAG1600.086.547781
GGTATCA3050.078.8563161
ATCTACC4150.058.89815145
GTGGTAT1006.85759E-1058.3058781
GCGGGTA1153.8198777E-1157.0331159
ATTGAAT553.135209E-453.005353
ATCAACG8200.050.662123
TGAGACA604.8165084E-448.5882344
TATAGGA752.4709905E-548.588232
TCAACGC8400.048.588234
TACATGG17150.046.7466962
GTACATG17650.046.661221
TGGGAAT1751.8189894E-1245.807556
ATAGATG3050.043.008586
GTATAAA1053.6852089E-641.6470571
CATGGGA9700.041.3250434
ACATGGG18450.040.6877063
AATAGAT3300.039.750355
TAGATGG3350.039.1570637
Produced by FastQC (version 0.11.5)