FastQCFastQC Report
Thu 2 Jun 2016
DRR028553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551220
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT16290.2955262871448786No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA9900.17960161097202568No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC9540.17307064330031566No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA9160.1661768440912884No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG9140.16581401255397119No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG9090.16490693371067813No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC8790.15946446065091976No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT8640.15674322412104058RNA PCR Primer, Index 26 (100% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.14730960415079278No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG7910.14349987300896194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7770.14096005224774139No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA7770.14096005224774139No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT7580.13751315264322775No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT7570.13733173687456912No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA7150.12971227459090745No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA6950.1260839592177352No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC6950.1260839592177352No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG6910.12535829614310076No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG6650.12064148615797685No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA6590.11955299154602517No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT6510.11810166539675629No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA6240.11320343964297376No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA6180.11211494503102208No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT5990.10866804542650847RNA PCR Primer, Index 26 (100% over 24bp)
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT5960.10812379812053263No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG5920.10739813504589818No Hit
TGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATAC5840.10594680889662929No Hit
TCCTAGTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAAT5820.10558397735931208No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA5800.10522114582199485No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA5710.10358840390406734No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC5600.1015928304488226No Hit
CATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGT5550.10068575160552956No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC5540.10050433583687095No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA5540.10050433583687095No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGA5530.10032292006821233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7150.0106.809211
GTGGTAT1400.0103.902841
GCTCTAG903.6379788E-1272.731991
GTACAGC300.001942167872.43513
TGGTATC2050.070.668392
CCTAACT1100.065.850098
ATGCGTC350.003578328362.0872234
TGCGTCG350.003578328362.0872235
CAGTGTA400.006070922654.326329
ACCGTGA553.2319914E-452.680078
GTATCAA18550.049.402861
ATCAACG18400.049.2086223
GTTGTAT604.8658543E-448.4879951
CATGGGA9150.048.2900664
TAGCGTG450.00967101648.2900669
TCTGTAC604.965016E-448.2900623
TCAACGC19050.047.529594
GAGTATT951.9311774E-645.7484784
TTGTATA657.366129E-444.5754472
ATAGATG3100.044.3957026
Produced by FastQC (version 0.11.5)