FastQCFastQC Report
Thu 2 Jun 2016
DRR028552_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028552_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531815
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13100.053.3877834
ATCAACG13700.051.0496333
CAACGCA15050.046.4704325
AACGCAG15950.044.761786
GTATCAA18050.042.3793261
ACGCAGA17350.040.729987
CGCAGAG17700.038.2731488
GGTATCA12050.038.088641
TATCAAC20300.035.8877222
GTACATG27000.031.5692351
CCGCGCG1504.261978E-529.1360139
TACATGG28700.027.92242
GTATACG2051.2432669E-524.876321
TCTCGGG16500.024.847439145
ACATGGG32550.024.6197513
TATACCG1500.001453046924.2840276
GTATAAC6250.023.3126661
ATACGGG3752.8921932E-1023.3126667
TACGGGC3503.3196557E-922.893348
GCAGAGT26450.022.8571769
Produced by FastQC (version 0.11.5)