FastQCFastQC Report
Thu 2 Jun 2016
DRR028552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531815
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19750.070.693871
GTATCAA31700.050.6968151
TCAACGC33600.046.778834
ATCAACG33750.046.5709343
CAACGCA35250.044.5891885
AACGCAG37350.042.082176
TAGATCG907.525969E-540.239865
TATCAAC39700.040.13852
ACGCAGA40700.038.4404377
CGCAGAG41000.037.805848
GTACATG29400.035.3701021
TACATGG31050.032.6584362
ACATGGG32000.029.1990493
GCAGAGT51350.029.0573319
GTGGTAT11450.026.6742021
TGGTATC11750.025.8904972
AACACCG2401.6121685E-624.1439155
CATGGGA15200.023.3497074
CAGAGTA49850.019.7607179
ATACCGC1950.00687946718.5722456
Produced by FastQC (version 0.11.5)