FastQCFastQC Report
Thu 2 Jun 2016
DRR028551_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028551_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701921
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18550.051.086354
ATCAACG19100.049.615283
GTATCAA22650.045.379151
GGTATCA14400.044.5477331
CAACGCA24850.037.8392755
AACGCAG26200.035.8895456
GTACATG40150.035.585741
TATCAAC27550.035.455912
CGCAGAG26450.033.6162268
ACGCAGA27350.033.0459337
TACATGG42600.032.5124322
TATACCG1702.8893373E-630.014335
ACATGGG45550.029.1265093
TATCCGA1050.007507517527.7700184
CCGTTCG1100.00943346326.4998429
TCTCGGG22550.026.133747145
CGACGAC6950.024.81766145
GAGTACA33650.024.479291
GTATAGG5750.024.0874711
GCAGAGT35650.023.3035099
Produced by FastQC (version 0.11.5)