FastQCFastQC Report
Thu 2 Jun 2016
DRR028551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701921
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.073.569661
GTATCAA37900.054.874391
TCAACGC40150.050.3339734
ATCAACG40400.050.201793
CAACGCA43050.046.94335
AACGCAG44650.045.4233446
TATCAAC48200.042.5286872
ACGCAGA49800.040.28967
CGCAGAG49850.040.1038868
GCAGAGT59050.031.770389
GTACATG38550.029.8040661
GTGGTAT12350.028.2629261
TACATGG41100.027.3169632
TGGTATC13250.025.1469042
GAGTACA34350.024.9803281
ACATGGG43400.024.8678993
AGTACAT33800.023.3588982
CAGAGTA58750.021.9459659
ACCGTCC1700.003110057721.3041218
ATGACCG2455.1527153E-520.6954335
Produced by FastQC (version 0.11.5)