FastQCFastQC Report
Thu 2 Jun 2016
DRR028550_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028550_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659029
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11850.048.589221
ATCAACG15400.047.3271563
TCAACGC16250.044.851584
GTATCAA19450.042.3438151
CAACGCA18350.040.1146665
AACGCAG20000.036.4385726
GTACATG32650.032.5912361
TATCAAC23850.032.3928072
ACGCAGA21650.031.9774447
CGCAGAG21550.031.785728
TACATGG35150.028.6144162
ACATGGG35050.026.4086883
TCTCGGG19100.024.466133145
AGACCGT2303.0201732E-522.1799986
GAGTACA28750.021.548261
ACGATCC7250.020.824118145
CATACGC1750.00354943320.823953
GTTATAC5950.020.8239483
GTACGGA2454.9161306E-520.822046
CGACGAC6750.020.23649145
Produced by FastQC (version 0.11.5)