FastQCFastQC Report
Thu 2 Jun 2016
DRR028550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659029
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.071.01951
GTATCAA32750.054.844891
ATCAACG35800.049.3598183
TCAACGC36100.048.749014
CAACGCA37600.046.996845
AACGCAG40500.043.6316386
TATCAAC41350.042.909882
ACGCAGA45900.038.3407257
CGCAGAG45800.038.108198
GTACATG34700.031.2253481
GCAGAGT54050.030.9515939
TACATGG38000.026.8721392
ACATGGG35450.026.3536153
TCTAACG1100.00972468626.3350433
GTGGTAT11900.025.6656821
TGGTATC12100.024.5394732
GAGTACA27800.021.1880361
CAGAGTA52200.020.9494769
CCGTGCA3901.2361852E-820.426549
TATACTG11550.020.0647935
Produced by FastQC (version 0.11.5)