FastQCFastQC Report
Thu 2 Jun 2016
DRR028549_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028549_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072316
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15800.054.0263634
ATCAACG15850.053.395633
CAACGCA18550.046.014815
CGCAGAG21200.039.5669368
AACGCAG22150.038.865476
ACGCAGA22850.037.0317427
GGTATCA15050.036.3581471
GTATCAA24550.035.36491
GTACATG37550.034.584941
TATCAAC26250.033.07462
TACATGG44050.027.825332
ACATGGG43050.025.760093
GCAGAGT32100.024.3129989
TCTCGGG30200.023.766048145
ATACGCC2953.6515848E-722.2574886
CGTGTCG3055.044167E-721.5229829
TCTATAC13300.021.3938983
CATGGGA20400.020.3855154
GAGTACA32100.020.2284181
GTATTAG21450.020.0678881
Produced by FastQC (version 0.11.5)