FastQCFastQC Report
Thu 2 Jun 2016
DRR028549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072316
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.069.568161
GTATCAA39450.054.3751871
TCAACGC38600.054.0379074
ATCAACG39450.052.873593
CAACGCA40550.051.4392935
AACGCAG43950.047.130336
TATCAAC48500.043.903482
ACGCAGA50050.041.241477
CGCAGAG50500.040.7305538
GTACATG38400.032.9491161
GCAGAGT61700.032.1631629
GTACGGA2351.2787496E-930.8194926
TACATGG43100.027.3907342
GACCGAC1100.00972232826.3366577
TAGTACG6900.024.1419354
ACATGGG44450.024.1147793
CATGGGA22500.021.8886894
GTATATA21300.021.5073411
GCGTCAG3508.842653E-820.6930874
GTATAGA11950.020.6888711
Produced by FastQC (version 0.11.5)