FastQCFastQC Report
Thu 2 Jun 2016
DRR028548_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028548_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129665
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17500.050.862444
ATCAACG18350.048.5064163
CAACGCA20250.043.9551965
AACGCAG21450.041.495176
GTATCAA25950.037.3929441
GGTATCA16200.036.929571
ACGCAGA24500.035.434417
CGCAGAG25150.034.225278
TATCAAC28450.032.0555422
GTACATG35100.030.9709531
GTACCGT1203.9129116E-430.3986156
ATACCGC2003.0843148E-729.1812787
TACATGG38300.025.7164152
TCTCGGG35900.025.389095145
GTACGGA2101.4822639E-524.3188926
AATCCGC2502.1600354E-623.3461386
ACATGGG39300.022.8343143
GCAGAGT36850.021.7739549
TATACCG2353.5361772E-521.7317756
CCGGTCG3406.038499E-821.4537499
Produced by FastQC (version 0.11.5)