FastQCFastQC Report
Thu 2 Jun 2016
DRR028548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2129665
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.063.0226481
TCAACGC35150.051.3023344
ATCAACG35250.051.156793
GTATCAA38250.050.7694661
CAACGCA37300.048.3452265
AACGCAG39500.046.0192686
TATCAAC44700.040.9898262
ACGCAGA44600.040.7569777
CGCAGAG45050.040.0283438
GTACATG36900.035.0845951
GCAGAGT58900.029.5093089
TACATGG39750.028.9683072
ACATGGG39400.027.019933
GTGGTAT17350.024.7329161
TGGTATC14800.024.4664732
TATACGT2953.919704E-722.094474
GAGTACA33750.019.8260781
TGTACCG2205.950033E-419.7511185
CATGGGG28100.019.071664
GTACGAC1900.005927349419.0580943
Produced by FastQC (version 0.11.5)