FastQCFastQC Report
Thu 2 Jun 2016
DRR028547_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028547_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835116
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.052.395281
ATCAACG21600.051.0178223
TCAACGC21650.050.94
GTATCAA24000.047.4364431
CAACGCA27100.040.6636545
GTACATG45550.038.772671
TATCAAC29500.037.3554232
AACGCAG29950.036.794166
TACATGG47100.036.721992
CGCAGAG29700.034.8865788
ACGCAGA31000.033.894357
ACATGGG52850.031.4839043
GTACGAC1357.7800493E-427.029313
CATGGGG39100.024.0775174
GTATAGG8350.022.7240451
GCAGAGT40850.022.684349
GAGTACA37500.021.6018241
GTATTAA12600.021.430381
TATACTG12650.021.3456765
CATGGGA22000.021.2302954
Produced by FastQC (version 0.11.5)