FastQCFastQC Report
Thu 2 Jun 2016
DRR028547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR028547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835116
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19100.071.5583341
GTATCAA34200.056.119471
ATCAACG36450.051.470853
TCAACGC36450.051.470854
CAACGCA40250.046.2515565
TATCAAC43100.044.2015572
AACGCAG43000.043.1251536
GTACATG42000.038.081071
CGCAGAG48000.038.029318
ACGCAGA48800.037.5543147
TACATGG46500.032.090272
GCAGAGT58150.030.1456559
TATAACG1255.1818904E-428.974712
ACATGGG51850.028.0806033
CCGTGCA6600.025.2431189
GCCGTGC6350.025.0962058
TAGATCG1450.001233088924.9781975
CCTATAC7400.023.4930083
GAGTACA38700.022.5427061
AGTACAT36500.022.2271752
Produced by FastQC (version 0.11.5)